ケミカルバイオロジー研究グループ（終了）

室井 誠 （むろい まこと）

研究の興味

学生時代の生理活性物質のスクリーニング実験をとおして、微生物が人の考えつかないような生理活性物質をたくさん作っていることを知りました。そのような化合物がどのようにして活性を発現しているかに興味を持って研究をつづけています。

学歴

昭和63年3月

東京大学 農学部 農芸化学科 卒業

平成5年3月

東京大学大学院 農学系研究科 博士課程 修了　博士（農学）

職歴

平成5年10月

理化学研究所入所 動物・細胞システム研究室　研究員

平成15年4月 - 平成27年3月

理化学研究所 長田抗生物質研究室 先任研究員・専任研究員

平成21年10月 - 平成23年3月

理化学研究所　ケミカルバイオロジー研究基盤施設　先任研究員

平成23年4月 - 平成25年3月

理化学研究所　ケミカルバイオロジー研究基盤施設　専任研究員

平成25年4月 -

理化学研究所 環境資源科学研究センター ケミカルバイオロジー研究グループ

所属学会

• 日本農芸化学会
• 日本癌学会
• 日本がん分子標的学会
• ケミカルバイオロジー学会

主要論文

1. Ikeda H, Muroi M, Kondoh Y, Ishikawa S, Kakeya H, Osada H, Imoto M.: Miclxin, a novel MIC60 inhibitor, induces apoptosis via mitochondrial stress in β-catenin mutant tumor cells.
   ACS Chem Biol, [Epub ahead of print] (2020) [ doi: 10.1021/acschembio.0c00381 ]
2. Sako K, Futamura Y, Shimizu T, Matsui A, Hirano H, Kondoh Y, Muroi M, Aono H, Tanaka M, Honda K, Shimizu K, Kawatani M, Nakano T, Osada H, Noguchi K, Seki M.: Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana.
   Sci Rep, 10(1): 8691 (2020) [ doi: 10.1038/s41598-020-65614-9 ]
3. Suvarna K, Honda K,Muroi M, Kondoh Y, Osada H, Watanabe N: Measurement of ATPase activity of valosin-containing protein/p97.
   Bio-protocol: , 10, e3516 (2020) [ doi: 10.21769/BioProtoc.3516 ]
4. Suvarna K, Honda K, Muroi M, Kondoh Y, Watanabe N, Osada H: Identification of target protein for bio-active small molecule using photo-cross linked beads and MALDI-TOF mass spectrometry.
   Bio-protocol: , 10, e3517 (2020) [ doi: 10.21769/BioProtoc.3517 ]
5. Nagasawa I, Muroi M, Kawatani M, Ohishi T, Ohba SI, Kawada M, Osada H.: Identification of a small compound targeting PKM2-regulated signaling using 2D gel electrophoresis-based proteome-wide CETSA.
   Cell Chem Biol, 27(2), 186-196.e4 (2020) [ doi: 10.1016/j.chembiol.2019.11.010 ]
6. Muroi M, Osada H. Proteomic profiling for target identification of biologically active small molecules using 2D DIGE. Methods in Molecular Biology, 1888, 127-139 (2019) [ doi: 10.1007/978-1-4939-8891-4_7 ]
7. Li J, Sun K, Muroi M, Gao L, Chang YT, Osada H, Xiang L, Qi J.: Cucurbitacin B induces neurogenesis in PC12 cells and protects memory in APP/PS1 mice. J Cell Mol Med, [Epub ahead of print] (2019) [ doi: 10.1111/jcmm.14514 ]
8. Subedi A, Muroi M, Futamura Y, Kawamura T, Aono H, Nishi M, Ryo A, Watanabe N, Osada H.: A novel inhibitor of tumorspheres reveals the activation of the serine biosynthetic pathway upon mitochondrial inhibition. FEBS Lett, 593(8): 763-776 (2019) [ doi: 10.1002/1873-3468.13361 ]
9. Cheng L, Muroi M, Cao S, Bian L, Osada H, Xiang L, Qi J.: 3β,23,28-Trihydroxy-12-oleanene 3β-caffeate from Desmodium sambuense-induced neurogenesis in PC12 cells mediated by ER stress and BDNF-TrkB signaling pathways. Mol Pharm, 16(4): 1423–1432 (2019) [ doi: 10.1021/acs.molpharmaceut.8b00939 ]
10. Suvarna K, Honda K, Muroi M, Kondoh Y, Osada H, Watanabe N.: A small-molecule ligand of valosin-containing protein/p97 inhibits cancer cell-accelerated fibroblast migration. J Biol Chem, 294(9): 2988-2996 (2019) [ doi: 10.1074/jbc.RA118.004741 ]
11. Futamura Y, Muroi M, Aono H, Kawatani M, Hayashida M, Sekine T, Nogawa T, Osada H.: Bioenergetic and proteomic profiling to screen small molecule inhibitors that target cancer metabolisms. Biochim Biophys Acta, 1867(1): 28–37 (2019) [ doi: 10.1016/j.bbapap.2018.06.001 ]
12. Khalid A, Takagi H, Panthee S, Muroi M, Chappell J, Osada H, Takahashi S.: Development of a terpenoid-production platform in Streptomyces reveromyceticus SN-593. ACS Synth Biol, 6(12): 2339-2349 (2017) [ doi: 10.1021/acssynbio.7b00249 ]
13. Robke L, Laraia L, Carnero Corrales MA, Konstantinidis G, Muroi M, Richters A, Winzker M, Engbring T, Tomassi S, Watanabe N, Osada H, Rauh D, Waldmann H, Wu YW, Engel J.: Phenotypic identification of a novel autophagy inhibitor chemotype targeting lipid kinase VPS34. Angew Chem Int Ed Engl, 56(28): 8153-8157 (2017) [ doi: 10.1002/anie.201703738 ]
14. Uesugi S, Muroi M, Kondoh Y, Shiono Y, Osada H, Kimura KI.: Allantopyrone A activates Keap1-Nrf2 pathway and protects PC12 cells from oxidative stress-induced cell death. J Antibiot, 70(4): 429-434 (2017) [ doi: 10.1038/ja.2016.99 ]
15. Kawatani M, Muroi M, Wada A, Inoue G, Futamura Y, Aono H, Shimizu K, Shimizu T, Igarashi Y, Takahashi-Ando N, Osada H.: Proteomic profiling reveals that collismycin A is an iron chelator. Sci Rep, 6: 38385 (2016) [ doi: 10.1038/srep38385 ]
16. Kawamura T, Kawatani M, Muroi M, Kondoh Y, Futamura Y, Aono H, Tanaka M, Honda K, Osada H.: Proteomic profiling of small-molecule inhibitors reveals dispensability of MTH1 for cancer cell survival. Sci Rep, 6: 26521 (2016) [ doi: 10.1038/srep26521 ]
17. Minegishi H, Futamura Y, Fukashiro S, Muroi M, Kawatani M, Osada H, Nakamura H.: Methyl 3-((6-methoxy-1,4-dihydroindeno[1,2-c]pyrazol-3-yl)amino)benzoate (GN39482) as a tubulin polymerization inhibitor identified by MorphoBase and ChemProteoBase profiling methods. J Med Chem, 58 (10): 4230-4241 (2015) [ doi: 10.1021/acs.jmedchem.5b00035 ]
18. Kawamura T, Kondoh Y, Muroi M, Kawatani M, Osada H.
    A small molecule that induces reactive oxygen species via cellular glutathione depletion.
    Biochem J 463, 53-63 (2014)
19. Futamura Y, Kawatani M, Muroi M, Aono H, Nogawa T, Osada H.
    Identification of a molecular target of a novel fungal metabolite, pyrrolizilactone, by phenotypic profiling systems.
    Chembiochem 14, 2456-63 (2013)
20. Futamura Y, Muroi M, Osada H.
    Target identification of small molecules based on chemical biology approaches.
    Molecular bioSystems 9, 897-914 (2013)
21. Osada H, Muroi M, Yoshida M.
    The chemistry/biology interface.
    Bioorg Med Chem 20, 1844-1845 (2012)
22. Sasazawa Y, Kanagaki S, Tashiro E, Nogawa T, Muroi M, Kondoh Y, Osada H, Imoto M.
    Xanthohumol impairs autophagosome maturation through direct inhibition of valosin-containing protein.
    ACS Chem Biol 7, 892-900 (2012)
23. Futamura Y, Kawatani M, Kazami S, Tanaka K, Muroi M, Shimizu T, Tomita K, Watanabe N, Osada H.
    Morphobase, an Encyclopedic Cell Morphology Database, and Its Use for Drug Target Identification.
    Chem Biol 19, 1620-1630 (2012).
24. Asami Y, Jang JH, Soung NK, He L, Moon DO, Kim JW, Oh H, Muroi M, Osada H, Kim BY, Ahn JS.
    Protuboxepin A, a marine fungal metabolite, inducing metaphase arrest and chromosomal misalignment in tumor cells.
    Bioorg Med Chem 20, 3799-3806 (2012)
25. Khan MM, Simizu S, Suzuki T, Masuda A, Kawatani M, Muroi M, Dohmae N, Osada H.
    Protein disulfide isomerase-mediated disulfide bonds regulate the gelatinolytic activity and secretion of matrix metalloproteinase-9.
    Exp Cell Res 318, 904-914 (2012)
26. Jang JH, Asami Y, Jang JP, Kim SO, Moon DO, Shin KS, Hashizume D, Muroi M, Saito T, Oh H, Kim BY, Osada H, Ahn JS.
    Fusarisetin A, an acinar morphogenesis inhibitor from a soil fungus, Fusarium sp. FN080326.
    J Am Chem Soc 133, 6865-6867 (2011)
27. Kawatani M, Takayama H, Muroi M, Kimura S, Maekawa T, Osada H.
    Identification of a small-molecule inhibitor of DNA topoisomerase II by proteomic profiling.
    Chem Biol 18, 743-751 (2011).
28. Wierzba K, Muroi M, Osada H.
    Proteomics accelerating the identification of the target molecule of bioactive small molecules.
    Curr Opin Chem Biol 15, 57-65 (2011)
29. Muroi M, Kazami S, Noda K, Kondo H, Takayama H, Kawatani M, Usui T, Osada H.
    Application of proteomic profiling based on 2D-DIGE for classification of compounds according to the mechanism of action.
    Chem Biol 17, 460-470 (2010)